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In Jung Kim, Hyeok-Jin Ko, Tae-Wan Kim, In-Geol Choi and Kyoung Heon Kim Characteristics of the binding of a bacterial expansin (BsEXLX1) to microcrystalline cellulose Biotechnology and Bioengineering 110

Article first published online: 18 SEP 2012 | DOI: 10.1002/bit.24719

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Binding characteristics of a bacterial expansin (BsEXLX1) to microcrystalline cellulose were analyzed in comparison with those of CtCBD3 that is a Type A carbohydrate-binding module. Binding parameters of the Langmuir isotherm of BsEXLX1 to Avicel, binding competition between BsEXLX1 and CtCBD3 for Avicel, and lack of binding activity to cellooligosaccharides revealed that the binding behavior of BsEXLX1 follows that of Type A CBMs. However, the binding capacities of cellulose and xylans for BsEXLX1 were significantly lower than those for CtCBD3.

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