Somasundar Ashok, Mugesh Sankaranarayanan, Yeounjoo Ko, Kyeung-Eun Jae, Satish Kumar Ainala, Vinod Kumar and Sunghoon Park Production of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae ΔdhaTΔyqhD which can produce vitamin B12 naturally Biotechnology and Bioengineering 110
Klebsiella pneumoniae showed potential to produce 3-hydroxypropionic acid (3-HP) in a significant amount. Carbon flux to 3-HP was critically controlled by oxygen in the opposite direction at two important nodes of glycerol (down) and 3-hydroxypropanealdehyde (up). Controlled aeration was necessary to produce 3-HP at a high amount. Recombinant strain KpBCΔdhaTΔyqhD overexpressing glycerol dehydratase and aldehyde dehydrogenase produced >28 g/L 3-HP at 5% DO in a bioreactor.
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