Verónica S. Martínez, Stefanie Dietmair, Lake-Ee Quek, Mark P. Hodson, Peter Gray and Lars K. Nielsen Flux balance analysis of CHO cells before and after a metabolic switch from lactate production to consumption Biotechnology and Bioengineering 110
The authors studied the metabolism of CHO cells before and after a metabolic switch from lactate production to consumption when glucose is depleted. The activity of TCA cycle remained the same despite the switch in carbon source, but the fraction of acetyl-CoA catabolized to CO2 increased from 3% to 32%. The metabolic model in this study was derived from a mouse genome scale model. Despite the model being underdetermined, the estimated flux ranges were sufficiently constrained to allow meaningful comparative analyses.
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