Meile Liu and Chetan T. Goudar Gene expression profiling for mechanistic understanding of cellular aggregation in mammalian cell perfusion cultures Biotechnology and Bioengineering 110
Aggregation of baby hamster kidney (BHK) cells cultivated in perfusion mode was studied by comparing expression profiles of 84 genes in the extracellular adhesion molecules (ECM) pathway by qRT-PCR. In aggregated cultures, 4 genes (COL1A1, COL4A1 THBS2, and VCAN) were consistently up-regulated while 2 (NCAM1 and THBS1) were consistently down-regulated. Based on the differential gene expression results, two mechanistic models were proposed for cellular aggregation. The first suggested cell aggregation was mediated by secreted thrombospondin, while collagen and fibronectin proteins, which have affinity for one another as well as binding sites for cells in culture, were implicated in the second model.
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