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Yang-Chun Yong, Yang-Yang Yu, Yun Yang, Jing Liu, Jing-Yuan Wang and Hao Song Enhancement of extracellular electron transfer and bioelectricity output by synthetic porin Biotechnology and Bioengineering 110

Version of Record online: 5 OCT 2012 | DOI: 10.1002/bit.24732

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A heterogeneously expressed synthetic porin OprF dramatically increased the membrane permeability of E. coli, resulting in a remarkable decrease in charge-transfer resistance, enhancement in extracellular electron transfer (EET). The oprF-expression strain can efficiently use riboflavin as the electron shuttle, however, its parental strain cannot. Thus, the oprF-expression strain delivered a much higher current output than its parent strain. The results substantiated the observation that membrane permeability can determine the usage spectrum of electron shuttles and the EET efficiency.

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