E-mail a Wiley Online Library Link

Sandra Cristea, Yevgeniy Freyvert, Yolanda Santiago, Michael C. Holmes, Fyodor D. Urnov, Philip D. Gregory and Gregory J. Cost In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration Biotechnology and Bioengineering 110

Article first published online: 23 OCT 2012 | DOI: 10.1002/bit.24733

Thumbnail image of graphical abstract

Targeted transgene integration is a critical tool for genome engineering. Unfortunately some organisms (including CHO and plant cells) are refractory to targeted transgenesis due to deficits in homology-based DNA repair. In this paper, Cristea and Freyvert, and coworkers develop a homology-independent targeted DNA integration technique based on zinc-finger nucleases that should allow for efficient targeted DNA integration in all organisms. Key to the method's success is linearization of the donor DNA in vivo by the zinc-finger nucleases.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field