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Florian Dismer, Sigrid Hansen, Stefan Alexander Oelmeier and Jürgen Hubbuch Accurate retention time determination of co-eluting proteins in analytical chromatography by means of spectral data Biotechnology and Bioengineering 110

Version of Record online: 23 OCT 2012 | DOI: 10.1002/bit.24738

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Maximizing the information extracted from elution peaks of co-eluting proteins in analytical chromatography can significantly reduce analysis times and help identify proteins in complex mixtures. This can be achieved by looking at spectral changes over elution time measured by standard DAD detectors and a subsequent de-convolution of the elution peaks, yielding the following information: (1) number of components in the peak, (2) accurate retention times, (3) pure component spectra, (4) rough estimate of the peak areas.

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