Se Jong Han, Sayeon Cho, Ky Lowehhaupt, So-Young Park, Sang Jun Sim and Yang-Gyun Kim Recombinant tagging system using ribosomal frameshifting to monitor protein expression Biotechnology and Bioengineering 110
Rapid and accurate measurement of expressed recombinant protein: The target gene was fused to the enhanced green fluorescent protein (EGFP) gene with a -1 ribosomal frame shifting (-1RFS) element. The unfused target and the target-EGFP fusion proteins were produced at a fixed ratio showing high enzymatic activity without a large metabolic burden. Furthermore, the expression ratio between unfused and fused forms was adjustable simply by exchanging -1RFS signals. This method would be useful for the real-time monitoring of recombinant protein expression.
Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf