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Se Jong Han, Sayeon Cho, Ky Lowehhaupt, So-Young Park, Sang Jun Sim and Yang-Gyun Kim Recombinant tagging system using ribosomal frameshifting to monitor protein expression Biotechnology and Bioengineering 110

Article first published online: 1 NOV 2012 | DOI: 10.1002/bit.24740

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Rapid and accurate measurement of expressed recombinant protein: The target gene was fused to the enhanced green fluorescent protein (EGFP) gene with a -1 ribosomal frame shifting (-1RFS) element. The unfused target and the target-EGFP fusion proteins were produced at a fixed ratio showing high enzymatic activity without a large metabolic burden. Furthermore, the expression ratio between unfused and fused forms was adjustable simply by exchanging -1RFS signals. This method would be useful for the real-time monitoring of recombinant protein expression.

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