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Alexandre Valancin, Balasubrahmanyan Srinivasan, Jean Rivoal and Mario Jolicoeur Analyzing the effect of decreasing cytosolic triosephosphate isomerase on Solanum tuberosum hairy root cells using a kinetic–metabolic model Biotechnology and Bioengineering 110

Article first published online: 18 OCT 2012 | DOI: 10.1002/bit.24747

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In this work, the authors studied the effects of a 90% decrease in cytosolic triosephosphate isomerase expression in Solanum tuberosum hairy roots engineered with a constitutively expressed antisense RNA. Experimental data and simulations from a kinetic-metabolic model were used to demontrate that antisense roots showed increased exchanges between the pentose phosphate and the glycolytic pathways, as well as increased oxygen uptake and growth rate.

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