Xiadi Gao, Rajeev Kumar, Jaclyn D. DeMartini, Hongjia Li and Charles E. Wyman Application of high throughput pretreatment and co-hydrolysis system to thermochemical pretreatment. Part 1: Dilute acid Biotechnology and Bioengineering 110
This study extended the application of a recently developed high throughput pretreatment and hydrolysis (HTPH) system originally developed for hydrothermal (water-only) pretreatment to dilute acid pretreatment. To neutralize the whole slurry resulting from dilute acid pretreatment and to maintain the optimum pH required for enzymes maximum activity, a novel single-step neutralization and buffering method was developed, as shown in the image, and was successfully applied for dilute acid pretreatment of several feedstocks at various pretreatment conditions showing the comparable results to conventional method.
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