E-mail a Wiley Online Library Link

Lauren Feeney, Veronica Carvalhal, X. Christopher Yu, Betty Chan, David A. Michels, Yajun Jennifer Wang, Amy Shen, Jan Ressl, Brendon Dusel and Michael W. Laird Eliminating tyrosine sequence variants in CHO cell lines producing recombinant monoclonal antibodies Biotechnology and Bioengineering 110

Article first published online: 15 FEB 2013 | DOI: 10.1002/bit.24759

Thumbnail image of graphical abstract

This work investigates a commonly observed sequence variant (SV) in several CHO cell produced monoclonal antibodies, and how the levels of key amino acids in the cell culture fermentation process can impact the level of SV. Specifically, several tyrosine residues throughout the protein were found to have either phenylalanine or histidine incorporated in place of tyrosine; it was determined that this event strongly correlated with tyrosine depletion. The data suggests that the mechanism of sequence variant formation is mRNA mistranslation.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field