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Zhenlin Han, Catherine Madzak and Wei Wen Su Tunable nano-oleosomes derived from engineered Yarrowia lipolytica Biotechnology and Bioengineering 110

Article first published online: 5 NOV 2012 | DOI: 10.1002/bit.24761

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This study demonstrates facile biosynthesis of functionalized oleosomes within oleaginous yeast Yarrowia lipolytica, by expressing oleosin fusion proteins which are specifically targeted to and anchored on the surface of the oleosomes. Engineered oleosomes having a mean diameter of 200–300 nm and with both cell-targeting and reporting activities were created and shown to be functional. The Yarrowia oleosome surface display system serves as a simple platform for creating novel “designer” nanoparticles.

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