Shu-Juan Gao, Jun-Qing Wang, Min-Chen Wu, Hui-Min Zhang, Xin Yin and Jian-Fang Li Engineering hyperthermostability into a mesophilic family 11 xylanase from Aspergillus oryzae by in silico design of N-terminus substitution Biotechnology and Bioengineering 110
The N-terminus of AoXyn11A to be replaced by the corresponding region of EvXyn11TS was selected by comparing B-factors between AoXyn11A and EvXyn11TS, and confirmed by comparing the total energy value of AEx11A with that of AoXyn11A. Based on the in silico design of N-terminus substitution, the AEx11A was constructed and expressed in P. pastoris. The temperature optimum of reAEx11A was 30°C higher than that of reAoXyn11A, and its half-life was 197-fold longer than that of reAoXyn11A at 70°C.
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