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Tom Craig and Andrew J. Daugulis Polymer characterization and optimization of conditions for the enhanced bioproduction of benzaldehyde by Pichia pastoris in a two- ;phase partitioning bioreactor Biotechnology and Bioengineering 110

Version of Record online: 23 NOV 2012 | DOI: 10.1002/bit.24778

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Pichia pastoris has high alcohol oxidase activity and, although often directed at methanol metabolism, can non-specifically oxidize other alcohols, here benzyl alcohol to benzaldehyde, an important fragrance/flavour compound. Benzaldehyde strongly inhibited the biotransformation, however, and a Two-Phase Partitioning Bioreactor (TPPB) was employed using amorphous polymer beads to sequester the produced benzaldehyde. The uptake mechanism was confirmed to be by absorption, not adsorption, as is seen in non-selective materials such as activated carbon. The benzaldehyde volumetric productivity was double in the TPPB compared to single phase.

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