Timothy Iskra, Glen R. Bolton, Jonathan L. Coffman and Ranga Godavarti The effect of protein a cycle number on the performance and lifetime of an anion exchange polishing step Biotechnology and Bioengineering 110
An understanding of the performance linkages between the unit operations can lead to robust manufacturing processes. In this study, a unique linkage between two chromatography unit operations is described. When material from a Protein A column that had been cycled a few times (<10) was loaded on the anion-exchange chromatography resin, premature loss of capacity was observed. Depth filtration of the Protein A peak pool was found to reduce the level of the foulant and significantly improve the AEX resin capacity, robustness and lifetime.
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