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Gargi Seth, Robert W. Hamilton, Thomas R. Stapp, Lisa Zheng, Angela Meier, Krista Petty, Stephenie Leung and Srikanth Chary Development of a new bioprocess scheme using frozen seed train intermediates to initiate CHO cell culture manufacturing campaigns Biotechnology and Bioengineering 110

Version of Record online: 4 JAN 2013 | DOI: 10.1002/bit.24808

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FASTEC (Frozen Accelerated Seed Train for Execution of a Campaign) approach involves initiating cell culture manufacturing campaigns with CHO cells cryopreserved in large volume bags (FROSTIs- Frozen Seed Train Intermediates). Each FROSTI thaw to initiate an inoculum train for a manufacturing campaign provides the same starting point and cell source for each run.

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