Patricia Bubner, Harald Plank and Bernd Nidetzky Visualizing cellulase activity Biotechnology and Bioengineering 110
Visualization is a powerful means to capture cellulase dynamics and to access the nano- and meso-scopic events occurring on the cellulose surface. In the last three decades, many visualization studies employing different methods such as transmission electron microscopy, atomic force microscopy, and optical microscopy, have greatly contributed to our current understanding of enzymatic cellulose hydrolysis. This review discusses methods and substrates used, pinpoints milestones, and gives an outlook on what can be expected from visualizing cellulase activity in the future.
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