Justin Eyquem, Laurent Poirot, Roman Galetto, Andrew M. Scharenberg and Julianne Smith Characterization of three loci for homologous gene targeting and transgene expression Biotechnology and Bioengineering 110
To identify potential integration sites for efficient transgene expression, the authors performed homologous gene targeting at three new loci chosen to represent different genomic environments. For clones carrying single copy insertions, they observed sustainable expression of a GFP cassette without disturbing the expression of flanking genes. Differences in expression were observed as a function of the locus, the promoter and the transgene orientation within the cassette but, the expression levels observed among individual clones with the same expression cassette at the same locus were highly reproducible and predictable.
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