Thomas Eixelsberger, John M. Woodley, Bernd Nidetzky and Regina Kratzer Scale-up and intensification of (S)-1-(2-chlorophenyl)ethanol bioproduction: Economic evaluation of whole cell-catalyzed reduction of o-Chloroacetophenone Biotechnology and Bioengineering 110
Reduction of 300 mM o-chloroacetophenone was accomplished with E. coli cells co-expressing Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase. Process boundaries with respect to biological, reaction and economic considerations were investigated in a ‘window of operation’ analysis. The operational space for economic (S)-1-(2-chlorophenyl)ethanol production was depicted a function of catalyst loading, product concentration, cell activity and substrate toxicity. Process scale-up and intensification extended the catalyst loading and economic product concentration limits and thereby shifted the process into the operational space.
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