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Bradley Niesner and Narendra Maheshri Using the cre–lox system to randomize target gene expression states and generate diverse phenotypes Biotechnology and Bioengineering 110

Version of Record online: 3 JUN 2013 | DOI: 10.1002/bit.24952

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By flanking promoters in budding yeast with inverted loxP sites and adding Cre recombinase to randomize their orientation, the authors demonstrate the ability to produce a combinatorial explosion (2N, 3N or 4N) of expression states with a small (N) number of genetic modifications. They show how this approach can reveal novel epistatic interactions in base excision repair and suggest how it may be deployed for biotechnological applications.

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