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Thomas C. Scanlon, Sarah M. Dostal and Karl E. Griswold A high-throughput screen for antibiotic drug discovery Biotechnology and Bioengineering 111

Version of Record online: 29 AUG 2013 | DOI: 10.1002/bit.25019

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Ultra-high-throughput screen for antibiotic drug discovery. A and B: Environmental DNA is cloned into a vector for heterologous expression in E. coli hosts. C: A microfluidic device is used to generate agarose-in-oil micro-emulsions in which individual recombinant E. coli are co-encapsulated with live bacterial pathogens. D: Droplets in which the recombinant clone secretes a biologic antibiotic are labeled with the SyTox Orange viability probe (orange circles). E: Droplet populations are sorted by FACS to isolate recombinant hosts secreting bactericidal natural products.

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