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Shin-ichiro M. Nomura, Kanta Tsumoto, Tsutomu Hamada, Kazunari Akiyoshi, Yoichi Nakatani and Kenichi Yoshikawa Gene Expression within Cell-Sized Lipid Vesicles ChemBioChem 4

Version of Record online: 3 NOV 2003 | DOI: 10.1002/cbic.200300630

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Cross-sectional images of a giant vesicle containing transcription/translation reaction solution have been obtained by confocal microscopy. The green signal indicates the expression of rsGFP within the vesicular space, while the red (hydrophobic fluorochrome) indicates the spherical vesicular membrane shape. In the early stage of the reaction, the expression efficiency inside the vesicle was remarkably higher than that in the solution outside.

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