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Ken Kasahara , Takanori Miyamoto, Takashi Fujimoto, Hiroki Oguri, Tetsuo Tokiwano , Hideaki Oikawa, Yutaka Ebizuka and Isao Fujii Solanapyrone Synthase, a Possible Diels–Alderase and Iterative Type I Polyketide Synthase Encoded in a Biosynthetic Gene Cluster from Alternaria solani ChemBioChem 11

Article first published online: 19 MAY 2010 | DOI: 10.1002/cbic.201000173

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Enzyme-catalyzed Diels–Alder cycloaddition: The biosynthetic gene cluster for solanapyrone has been cloned from Alternaria solani. The polyketide synthase product was identified as desmethylprosolanapyrone I. Expression and in vitro analysis indicated that Sol5—solanapyrone synthase—is a possible enzyme that catalyzes Diels–Alder cycloaddition.

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