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Dr. Sarah E. Lee, Dr. Lucy M. Elphick, Dr. Holger B. Kramer, Dr. Alexandra M. E. Jones, Dr. Emma S. Child, Dr. Alexandra A. Anderson, Dr. Laurent Bonnac, Dr. Natsuko Suwaki, Dr. Benedikt M. Kessler, Prof. Dr. Véronique Gouverneur and Dr. David J. Mann The Chemoselective One-Step Alkylation and Isolation of Thiophosphorylated Cdk2 Substrates in the Presence of Native Cysteine ChemBioChem 12

Version of Record online: 8 FEB 2011 | DOI: 10.1002/cbic.201000528

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It's a kinase magic: The identification of protein substrates is hampered by the large number of kinases active in cells at a given time. Here we use a chemical genetic approach to specifically label the substrates of an analogue-sensitive kinase with thiophosphate and use pH-dependent S-alkylation to selectively recover thiophosphorylated peptides.

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