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Akimasa Yoshimura, Dr. Shin Mizukami, Dr. Yuichiro Hori, Shuji Watanabe and Prof. Kazuya Kikuchi Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β-Lactamase-Tag Technology ChemBioChem 12

Version of Record online: 18 MAR 2011 | DOI: 10.1002/cbic.201100021

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Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs).

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