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Peter Siman, Ofrah Blatt, Tal Moyal, Dr. Tsafi Danieli, Dr. Mario Lebendiker, Prof. Hilal A. Lashuel, Prof. Assaf Friedler and Prof. Ashraf Brik Chemical Synthesis and Expression of the HIV-1 Rev Protein ChemBioChem 12

Version of Record online: 12 APR 2011 | DOI: 10.1002/cbic.201100033

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Winning HIV-1 Rev: The protein HIV-1 Rev plays an important role in the HIV lifecycle; however, its high tendency to aggregate has hindered several studies that aimed at deciphering better its structure and function. Two highly reproducible methods to generate this protein in large quantities, based on chemical synthesis and recombinant expression, are presented.

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