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Dr. Peter B. Crowley, Elysian Chow and Tatiana Papkovskaia Protein Interactions in the Escherichia coli Cytosol: An Impediment to In-Cell NMR Spectroscopy ChemBioChem 12

Article first published online: 29 MAR 2011 | DOI: 10.1002/cbic.201100063

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Electrostatic interactions in the cytosol: Size-exclusion chromatography was used to demonstrate binding between cytochrome c and E. coli cytosolic proteins. High concentrations of NaCl disrupted complex formation, but the physiologically relevant salt KGlu was less effective. Charge-inverted mutants also disrupted the interaction. In contrast to the wild-type protein, a triple mutant could be detected in cell lysates by NMR spectroscopy.

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