Rosalina Wisastra, Prof. Dr. Klaas Poelstra, Prof. Dr. Rainer Bischoff, Dr. Harm Maarsingh, Prof. Dr. Hidde J. Haisma and Dr. Frank J. Dekker Antibody-Free Detection of Protein Tyrosine Nitration in Tissue Sections ChemBioChem 12
Inflamed proteins: Tyrosine nitration is a covalent post-translational protein modification that represents a biomarker for inflammatory diseases. We introduce a novel method to detect nitrotyrosine in biological samples by chemoselective conversion into a fluorophore. We describe the applicability of this methodology to detect protein-bound nitrotyrosine by fluorescence microscopy in tissue sections and on Western blot membranes.
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