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Anaëlle Dumas and Prof. Dr. Nathan W. Luedtke Fluorescence Properties of 8-(2-Pyridyl)guanine “2PyG” as Compared to 2-Aminopurine in DNA ChemBioChem 12

Version of Record online: 22 JUL 2011 | DOI: 10.1002/cbic.201100214

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Glowing DNA: Here we report a comparison of 8-(2-pyridyl)-2′-deoxyguanosine (2PyG) with the common fluorescent base analogue 2-aminopurine (2AP). 2PyG exhibits enhanced fluorescence quantum yields upon its incorporation into nucleic acids (ϕ=0.03, ε=20 000 cm−1M−1, vs. ϕ=0.002, ε=5600 cm−1M−1 for 2AP), and it can be used to quantify structure-specific energy-transfer efficiencies.

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