Prof. Dr. Yoshihiro Yamaguchi, Prof. Dr. Shijia Ding, Emi Murakami, Kayo Imamura, Sachiko Fuchigami, Ryo Hashiguchi, Prof. Dr. Katsuhide Yutani, Dr. Hiromasa Mori, Prof. Dr. Shinnichiro Suzuki, Prof. Dr. Yoshichika Arakawa and Prof. Dr. Hiromasa Kurosaki A Demetallation Method for IMP-1 Metallo-β-Lactamase with Restored Enzymatic Activity Upon Addition of Metal Ion(s) ChemBioChem 12
Article first published online: 7 JUL 2011 | DOI: 10.1002/cbic.201100342
Think zinc: We have developed a new preparation method for generating the apo-enzyme of IMP-1 metallo-β-lactamase (apo-IMP-1) with the combination of a chelating agent (EDTA) and desalting column chromatography. An advantage of this method is that enzymatic activity of the apo-enzyme comparable to that observed with the native enzyme could be restored upon addition of ZnII ions (see scheme).
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