Si Jia Pan, Jakub Rajniak, Wai Ling Cheung and Prof. A. James Link Construction of a Single Polypeptide that Matures and Exports the Lasso Peptide Microcin J25 ChemBioChem 13
Roped in: The lasso peptide microcin J25 (MccJ25) is matured by two enzymes and is exported by a putative ABC transporter (see graphic). We probed the function of the maturation enzymes using mutagenesis. We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25.
Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf