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Si Jia Pan, Jakub Rajniak, Wai Ling Cheung and Prof. A. James Link Construction of a Single Polypeptide that Matures and Exports the Lasso Peptide Microcin J25 ChemBioChem 13

Version of Record online: 23 DEC 2011 | DOI: 10.1002/cbic.201100596

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Roped in: The lasso peptide microcin J25 (MccJ25) is matured by two enzymes and is exported by a putative ABC transporter (see graphic). We probed the function of the maturation enzymes using mutagenesis. We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25.

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