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Dr. Justin D. Cohen, Peng Zou and Prof. Alice Y. Ting Site-Specific Protein Modification Using Lipoic Acid Ligase and Bis-Aryl Hydrazone Formation ChemBioChem 13

Version of Record online: 11 APR 2012 | DOI: 10.1002/cbic.201100764

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Two-step labeling: Lipoic acid ligase (W37I mutant) conjugates aryl aldehyde and aryl hydrazine substrates to recombinant proteins. Subsequent bis-aryl hydrazone formation targets fluorophores or fluorescent proteins to these proteins in vitro or on the surface of living cells.

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