Annika Borrmann, Sigrid Milles, Tilman Plass, Jan Dommerholt, Jorge M. M. Verkade, Prof. Dr. Manfred Wießler, Dr. Carsten Schultz, Prof. Dr. Jan C. M. van Hest, Dr. Floris L. van Delft and Dr. Edward A. Lemke Genetic Encoding of a Bicyclo[6.1.0]nonyne-Charged Amino Acid Enables Fast Cellular Protein Imaging by Metal-Free Ligation ChemBioChem 13
Version of Record online: 3 SEP 2012 | DOI: 10.1002/cbic.201200407
Quick labeling: A bicyclo[6.1.0]nonyne-conjugated lysine has been synthesized and genetically encoded into proteins. This new unnatural amino acid provides a tool for fast and selective labeling in vitro and inside mammalian cells with both azido- and tetrazine-functionalized dyes.
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