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Christian M. Cole, Dr. Jun Yang, Dr. Jolita Šečkutė and Prof. Neal K. Devaraj Fluorescent Live-Cell Imaging of Metabolically Incorporated Unnatural Cyclopropene-Mannosamine Derivatives ChemBioChem 14

Version of Record online: 4 JAN 2013 | DOI: 10.1002/cbic.201200719

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Sugar coated: We recently developed methylcyclopropenes as low-molecular-weight tetrazine coupling partners. Here, we demonstrate that methylcyclopropenes can meet the stringent steric demands required for metabolic imaging of unnatural mannosamines on live cells. Using sequential azide–alkyne chemistry, we also demonstrate multicolor imaging of two different metabolically incorporated unnatural sugars.

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