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Prof. Kaori Sakurai, Rika Yamada, Ayumi Okada, Masaki Tawa, Shimpei Ozawa and Maia Inoue Selective Fluorescence Detection of Small-Molecule-Binding Proteins by Using a Dual Photoaffinity Labeling System ChemBioChem 14

Article first published online: 1 FEB 2013 | DOI: 10.1002/cbic.201200758

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Photo opportunity: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co-reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.

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