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Dr. Jong-Wha Jung, Sarah K. Edwards and Dr. Eric T. Kool Selective Fluorogenic Chemosensors for Distinct Classes of Nucleases ChemBioChem 14

Version of Record online: 1 FEB 2013 | DOI: 10.1002/cbic.201300001

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Nuclease sensor trio: Fluorogenic DNA sensors were developed for distinct classes of nucleases: 3′-exonucleases, 5′-exonucleases, and endonucleases. The highly selective sensors, built from very small modified DNA oligomers containing the unnatural fluorescent base pyrene, and employing thymine as a quencher, were found to function in a variety of complex biological media.

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