Marcus Schallmey, Robert J. Floor, Prof. Dr. Bernhard Hauer, Dr. Michael Breuer, Peter A. Jekel, Dr. Hein J. Wijma, Prof. Dr. Bauke W. Dijkstra and Prof. Dr. Dick B. Janssen Biocatalytic and Structural Properties of a Highly Engineered Halohydrin Dehalogenase ChemBioChem 14
Synergistic mutations: A halohydrin dehalogenase with 37 mutations and improved catalytic properties for statin side chain synthesis has been biochemically characterized. Crystal structures with different ligands in the active site give insight into the way in which individual mutations contribute to enhanced stability and faster cyanolysis of epoxides and illustrate the importance of synergistic mutations in directed evolution.
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