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Dr. Dorothea Pinotsi, Dr. Alexander K. Buell, Prof. Dr. Christopher M. Dobson, Dr. Gabriele S. Kaminski Schierle and Prof. Dr. Clemens F. Kaminski A Label-Free, Quantitative Assay of Amyloid Fibril Growth Based on Intrinsic Fluorescence ChemBioChem 14

Article first published online: 16 APR 2013 | DOI: 10.1002/cbic.201300103

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Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein (blue curve) but not when α-synuclein seeds are incubated with β-synuclein monomeric protein (black curve), thus showing that no seeded growth occurred in this case.

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