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Pavel Kielkowski, Nelson L. Brock, Dr. Jeroen S. Dickschat and Prof. Dr. Michal Hocek Nucleobase Protection Strategy for Gene Cloning and Expression ChemBioChem 14

Version of Record online: 26 MAR 2013 | DOI: 10.1002/cbic.201300127

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Protecting group chemistry meets molecular biology: Chemically modified dATP carrying a bulky triethylsilylethynyl group was used in a PCR-based synthesis of a gene internally protected against cleavage by restriction endonucleases. The unmodified flanking regions were cleaved for cloning into a plasmid which was replicated by E. coli, and used for protein production.

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