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Dr. Kwon-Young Choi, Eun-Ok Jung, Prof. Dr. Hyungdon Yun, Prof. Dr. Yung-Hun Yang, Prof. Dr. Romas J. Kazlauskas and Prof. Dr. Byung-Gee Kim Development of Colorimetric HTS Assay of Cytochrome P450 for ortho-Specific Hydroxylation, and Engineering of CYP102D1 with Enhanced Catalytic Activity and Regioselectivity ChemBioChem 14

Article first published online: 18 JUN 2013 | DOI: 10.1002/cbic.201300212

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High-speed activity: A general HTS assay method for ortho-specific hydroxylation by P450 enzymes is presented. In order to screen for P450 variants with higher hydroxylation activities, an indirect HTS assay method to sense aldehyde molecules generated from O-dealkylation reaction of chemically permethylated substrate was developed.

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