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Maria Svedendahl, Biljana Jovanović, Linda Fransson and Per Berglund Suppressed Native Hydrolytic Activity of a Lipase to Reveal Promiscuous Michael Addition Activity in Water ChemCatChem 1

Version of Record online: 11 AUG 2009 | DOI: 10.1002/cctc.200900041

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Enzyme catalytic promiscuity: The native hydrolytic and promiscuous Michael addition activities of Pseudozyma antarctica lipase B, formerly known as Candida antarctica lipase B, are explored with and without the nucleophilic Ser 105 residue in the active site by using both laboratory experiments and molecular modeling (see picture).

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