Dr. Anders Hamberg, Dr. Anders O. Magnusson, Francis J. Hu and Prof. Karl Hult Selective Monoacylation of Diols by Substrate Assisted Catalysis in T40A Candida antarctica Lipase B ChemCatChem 5
Transition state mutates! Deletion of one hydroxyl group in the oxyanion hole through T40A-mutation of lipase B from Candida antarctica enables substrate-assisted catalysis of diols. The hydroxyl group that is not attacking the acyl enzyme can play the role of the deleted hydroxyl group in the oxyanion hole and restore stabilization of the transition state. The monoester formed cannot react further to form the diester, which increases the monoester yield.
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