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John V. Simpson, Marissa Burke and Renee D. Jiji Application of EEM fluorescence in combination with PARAFAC analysis to simultaneously monitor quercetin in its deprotonated, aggregated, and protein bound states Journal of Chemometrics 25

Version of Record online: 1 SEP 2010 | DOI: 10.1002/cem.1325

Excitation-emission matrix (EEM) fluorescence in combination with weighted-parallel factor analysis (WPARAFAC) was employed to characterize the pH and concentration dependent fluorescence of aqueous quercetin. Distinct pH and concentration dependent fluorescence profiles were observed. The fluorescence of quercetin was also characterized in the presence of bovine serum albumin, which resulted in a narrowing and red shift and of the excitation maxima. Coupling WPARAFAC and EEM fluorescence, enabled simultaneous detection of multiple fluorescent quercetin species in solution.

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