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Hideo Takakusa, Kazuya Kikuchi, Yasuteru Urano, Hirotatsu Kojima and Tetsuo Nagano A Novel Design Method of Ratiometric Fluorescent Probes Based on Fluorescence Resonance Energy Transfer Switching by Spectral Overlap Integral Chemistry - A European Journal 9

Article first published online: 13 MAR 2003 | DOI: 10.1002/chem.200390167

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A novel design method of ratiometric fluorescent probes has been developed using fluorescence resonance energy transfer switching by spectral overlap integral. Based on this approach, we succeeded in developing novel fluorescent probes for protein tyrosine phosphatase (see scheme).

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