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Manuela Avi, Romana M. Wiedner, Herfried Griengl and Helmut Schwab Improvement of a Stereoselective Biocatalytic Synthesis by Substrate and Enzyme Engineering: 2-Hydroxy-(4′-oxocyclohexyl)acetonitrile as the Model Chemistry - A European Journal 14

Version of Record online: 12 NOV 2008 | DOI: 10.1002/chem.200800609

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Precision engineering: The improvement of a stereoselective synthesis, catalyzed by hydroxynitrile lyase, by substrate and enzyme engineering is described. The ee value of this catalysis was increased from 10 % to 90 % by using 20 times less enzyme. HNL=hydroxynitrile lyase.

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