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Kazuhisa Hirabayashi, Dr. Kenjiro Hanaoka, Dr. Manabu Shimonishi, Takuya Terai, Dr. Toru Komatsu, Dr. Tasuku Ueno and Prof. Dr. Tetsuo Nagano Selective Two-Step Labeling of Proteins with an Off/On Fluorescent Probe Chemistry - A European Journal 17

Article first published online: 22 NOV 2011 | DOI: 10.1002/chem.201102664

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Beaming up: A fluorescent probe with a nitrilotriacetic acid (NTA)-Ni2+ hexahistidine (His)-binding moiety and a 2,4-dinitrophenyl ether quencher moiety was found to have off/on fluorescence change owing to proximity-enhanced quencher cleavage on binding to a cysteine-modified His-tag (see scheme).

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