E-mail

E-mail a Wiley Online Library Link

Dr. Jacek Cieślak, Dr. Cristina Ausín, Dr. Andrzej Grajkowski and Dr. Serge L. Beaucage The 2-Cyano-2,2-dimethylethanimine-N-oxymethyl Group for the 2′-Hydroxyl Protection of Ribonucleosides in the Solid-Phase Synthesis of RNA Sequences Chemistry - A European Journal 19

Version of Record online: 18 FEB 2013 | DOI: 10.1002/chem.201204235

Thumbnail image of graphical abstract

Novel 2-RNA protection: The reaction of 2-cyano-2-methyl propanal with 2′-O-aminooxymethylribonucleosides leads to 2′-O-(2-cyano-2,2-dimethylethanimine-N-oxymethyl)ribonucleosides, which were then converted into their fully protected phosphoramidite derivatives (see scheme; DMTr=4,4′-dimethoxytrityl). These amidites were successfully employed in the solid-phase synthesis of three chimeric RNA sequences, each with a different purine/pyrimidine ratio. This approach is straightforward, efficient, and cost effective.

Complete the form below and we will send an e-mail message containing a link to the selected article on your behalf

Required = Required Field

SEARCH