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Tobias Vallon, Matthias Glemser, Dr. Sumire Honda Malca, Dr. Daniel Scheps, Dr. Joachim Schmid, Dr. Martin Siemann-Herzberg, Prof. Dr. Bernhard Hauer and Prof. Dr. Ralf Takors Production of 1-Octanol from n-Octane by Pseudomonas putida KT2440 Chemie Ingenieur Technik 85

Version of Record online: 25 APR 2013 | DOI: 10.1002/cite.201200178

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A two-phase biotransformation process for selective hydroxylation of n-octane to 1-octanol via heterologous expression of P450 monooxygenase in P. putida KT2440 is established under safe, industry-like bioreactor conditions. Based on topological studies potential limitations of NAD(P)H and ATP supply at production conditions could be excluded.

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