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Esmaeel R. Dadashzadeh, Matthew Hobson, L. Henry Bryant Jr, Dana D. Dean and Joseph A. Frank Rapid spectrophotometric technique for quantifying iron in cells labeled with superparamagnetic iron oxide nanoparticles: potential translation to the clinic Contrast Media & Molecular Imaging 8

Article first published online: 29 OCT 2012 | DOI: 10.1002/cmmi.1493

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A practical and accurate rapid spectrophotometric technique to quantify iron concentration in SPIO-labeled cells has been developed. The technique protocol lyses cells with 10% SDS in 1 min, does not require overnight acid digestion of SPIO, and measures colloidal iron directly using readily available UV–vis spectrophotometers. This protocol has comparable accuracy and limits of quantification to ICP-MS, NMR-R2 relaxometry and other colorimetric methods and can be applied to other metal nanoparticles (e.g. gold) used for labeling cells.

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