Takashi Ieki, Shinji Okada, Yoshiko Aihara, Makoto Ohmoto, Keiko Abe, Akihito Yasuoka and Takumi Misaka Transgenic labeling of higher order neuronal circuits linked to phospholipase C-β2–expressing taste bud cells in medaka fish Journal of Comparative Neurology 521
We generated transgenic medaka fish expressing a neuronal tracer, wheat germ agglutinin (WGA), under the control of the plc-β2 gene regulatory region to analyze the neuronal circuit connected to the taste sensory cells. WGA proteins expressed in the plc-β2-expressing taste bud cells (purple) were transported to a population of neurons in several ganglia and nuclei of the hindbrain at 12 days post fertilization (red), and in several brain regions including the diencephalon and telencephalon in fish that were 3 (orange) and 9 (green) months old. For abbreviations, see list in the text.
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